FIG 2.

Phosphonate-related gene clusters in Lake Matano metagenomic data. Contig00268 has high identity (66%) to the gene cluster encoding the multienzyme C-P lyase complex, phnIJKLM, from Rhodococcus opacus strains PD630 and R7 (only R. opacus strain PD630 is shown) and organization similar to that of the phn gene cluster from E. coli, whose C-P lyase pathway has been well studied (50, 67). Genes are colored based on their functions: transporter genes (phnCDE) are light gray, regulatory genes (phnFO) are white, phosphonate degradation genes (phnGHIJKLM) are dark gray, and the phnNP accessory genes are black.