TABLE 4.
Relative expression levels of phnJ expression in isolates grown under different conditions
| Phase | P sourcea | Avg ± SD of relative levels of phnJ transcripts |
|||
|---|---|---|---|---|---|
| LM-1 | LM-5 | LM6-1 | LM-Y | ||
| Exponential growth | Phosphate | 1.00 | 1.00 | 1.00 | 1.00 |
| MPn + phosphate | 13.92 ± 6.05 | 9.97 ± 1.77 | 13.75 ± 1.09 | 1.35 ± 0.32 | |
| MPn | 68.26 ± 9.94 | 32.50 ± 3.33 | 64.90 ± 11.16 | 261.81 ± 45.67 | |
| Stationary | Phosphate | 0.35 ± 0.06 | 0.06 ± 0.01 | 0.19 ± 0.06 | 0.17 ± 0.01 |
| MPn + phosphate | 0.6 ± 0.01 | 0.54 ± 0.21 | 0.52 ± 0.04 | 0.17 ± 0.03 | |
| MPn | 0.94 ± 0.32 | 1.60 ± 0.28 | 0.70 ± 0.04 | 1.36 ± 0.06 | |
a
Relative levels of phnJ transcripts in cells grown with phosphate (0.2 mM), phosphate + MPn (0.2 mM each), or MPn alone (0.2 mM) were determined, normalized based on total RNA, and compared to levels in cells grown on phosphate during exponential growth phase. For each sample, two biological replicates and two technical replicates were analyzed.