Figure 5.

The non-resolving inflammatory monocyte adaptation is independent of MyD88, but dependent on TRAM and TRIF. (A–L) Total RNAs were harvested from LPS-treated monocytes with MyD88, TRIF, and TRAM deficiency. Real-time PCR was performed to determine the expression levels of CCR5 (A,E,L), IL-12 (B,F,J), ARG1 (C,G,K), and iNOS (D,H,L). Data are representative of three separate experiments (error bar represent SEM, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, as compared to non-treated control group, Student’s t-test).