Skip to main content
. 2016 Jun 6;17(8):1265–1275. doi: 10.1111/mpp.12402

Figure 6.

Figure 6

Exogenous application of synthetic Rotylenchulus reniformis CEP1.1 domain peptide affects root development in a dose‐dependent manner. (A) Synthetic peptides corresponding to the C‐terminally‐encoded peptide (CEP) domains of RrCEP1.1 (R. reniformis, R. ren), AtCEP5 (Arabidopsis thaliana, A. tha), MhCEP2 (Meloidogyne hapla, M. hap) and MtCEP1.1 (Medicago truncatula, M. tru) (1 μm) significantly reduce the primary root length of A. thaliana compared with a randomized RrCEP1.1 (Rand) or a no‐peptide control (No pep) at 15 days post‐germination (n = 21–27, lower case letters indicate homogeneous subsets, P < 0.001 accounting for multiple t‐tests; error bars indicate standard error of the mean). (B) Representative primary root length at 15 days post‐germination. (C) Increasing concentrations of both RrCEP1.1 and AtCEP5 increase the magnitude of the response. No response is observed for the randomized peptide, even at 10 μm. Combined application of RrCEP1.1 and AtCEP5 at equimolar concentrations (red, 1 μm each) does not reduce the effect of AtCEP5, suggesting that R. reniformis CEPs are not refractory and do not block the CEP receptor when in competition with endogenous plant CEPs, but function similarly to reduce the cell division rate. (D) RrCEP1.1 and MtCEP1.1, but not MhCEP2, reduce the lateral root number on Medicago truncatula compared with wild‐type (n = 16–23, lower case letters indicate homogeneous subsets, P < 0.002 accounting for multiple t‐tests; error bars indicate standard error of the mean).