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PfEMP1 surface exposure is not impaired upon PFE1605w depletion in knobs. Determination of PfEMP1 surface exposure by detecting trypsin cleavage in 3D7 wild type, PFE1605wON/PFE1605wOFF and PFE1605w+RAP/PFE1605w−RAP parasites.
A. PFE1605wON/PFE1605wOFF.
B. PFE1605w+RAP/PFE1605w−RAP parasites. Trophozoites of each parasite line were treated with trypsin (+) or without trypsin (−), and the extracts were analysed by Western blot by using antibodies against ATS domains.
