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. 2016 Apr 26;18(10):1415–1428. doi: 10.1111/cmi.12583

Figure 5.

Figure 5

PFE1605w directly binds the ATS C‐terminus.

A. Fluorescence polarization titrations of 5‐FAM‐labelled ATS‐C constructs from PfEMP1 variants (Fig. S4A) with unlabelled PFE1605w PHIST domain. Data points, normalized to the fraction of ATS‐C bound at each PFE1605w concentration, are shown as coloured circles. The error bars were derived from four technical replicates. The solid lines correspond to data fitted with a single‐site association model. Equilibrium dissociation constants (K d) for the PFE1605w–ATS‐C interaction are shown. The interaction of PfEMP1 variant PFD0615c with PFE1605w could not be fitted.

B. Schematic representation of the mini‐PfEMP1 construct for Co‐IP experiments. C and D. LC‐ESI‐MS/MS results of two independent Co‐IP experiments using parasites expressing mini‐PfEMP1 constructs composed of the C‐terminal part of PF08_0141 (C) or PFF0010w (D).

Only peptide hits detected in both of the duplicate experiments are shown. Samples were also analysed by Western blot with α‐HA and α‐PFE1605w antibodies. IN, input; SN, supernatant; W, wash; E, elution.