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. 2016 Jun 20;14(12):2276–2287. doi: 10.1111/pbi.12581

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© 2016 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Gene constructs used for tobacco and poplar transformation. LB: left border sequence of T‐DNA. RB: right border sequence of T‐DNA. tNOS: nopaline synthase terminator. NptII: neomycin phosphotransferase gene. pNOS: nopaline synthase gene promoter sequence. 35S: cauliflower mosaic virus 35S gene promoter sequence. GUS: the coding sequence for the β‐glucuronidase gene. fPTAG2I: the forward orientation of the second intron of P. trichocarpa AGAMOUS (AG) 2 gene. Min35S: 60 basepairs of the 35S gene leader and promoter sequence that has no promoter activity. rPTAG2I: the reverse orientation of the second intron sequence of the P. trichocarpa AGAMOUS (AG) 2 gene. DT‐A: the coding sequence for the Diphtheria toxin A (DT‐A) gene, which codes for a ribosome inactivating protein.