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. 2016 Nov 10;6:36840. doi: 10.1038/srep36840

Figure 4. Shedding of vaccine viruses and induction of CnBV-2-reactive antibodies after vaccination of canaries with NDV and MVA vector vaccines (experiment 3).

Figure 4

(a) A group of 13 canaries (vaccine group) was vaccinated with a mixture of rNDV/CnBV-2/N and rNDV/CnBV-2/P and booster-vaccinated twice with rMVA/CnBV-2/N and rMVA/CnBV-2/P at days 14 and 28 after priming with NDV, while a second group of 13 birds (control group) received the respective parenteral vaccine strains. (b,c) Combined pharyngeal and cloacal swabs were collected at the indicated time points. CnBV-2 P RNA originating from rNDV/CnBV-2/P (b) or CnBV-2 P DNA from rMVA/CnBV-2/P (c) was quantified by qPCR assays. (d) Plasma samples were collected at the indicated time points and tested for the presence of CnBV-2-reactive antibodies by iIFT. The positions of the X axes indicate the detection limit of the respective test.