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. Author manuscript; available in PMC: 2016 Nov 10.
Published in final edited form as: Cell Rep. 2016 Feb 11;14(7):1698–1709. doi: 10.1016/j.celrep.2016.01.040

Figure 1. MiR-980 inhibition enhances olfactory memory by potentiating memory acquisition and stability.

Figure 1

(A) MiR-980 inhibition enhances 3h aversive memory. The miR-980 targeting sponge contains sequence mismatches to prevent RNA interference-mediated degradation of the sponge RNA (Ebert et al., 2007). Two independent UAS-miR-980SP insertions, in the attP40 (2nd chromosome) and attP2 (3rd chromosome) sites, and the hypomorphic miR-980 mutant NP3544 improve 3h memory. The UAS-miR-980 sponge expression was driven by the pan-neuronal c155-GAL4 element; transgenic lines containing scrambled sequences (UAS-scrambled) were used as controls. PI: performance index. Statistics: Experimental and control groups were compared by the two-tailed, two-sample Student’s t-test. p<0.01 for attP40 and NP3544 line, p<0.05 for attP2 insert. PIs are the mean ± SEM (standard error of the mean) with n≥6.

(B) MiR-980 inhibition retards memory decay. MiR-980 inhibition enhances 3min, 1h, and 3h memory. Three min, 1, 3, 9 and 24h memory was tested for UAS-miR-980SP and UAS-scrambled flies containing the c155-GAL4 driver. Statistics: The c155-GAL4>UAS-miR-980SP PI at each time point was compared to the c155-GAL4>UAS-scrambled PI on different days and compared using a two-tailed, two-sample Student’s t-tests for each time point. p<0.05. PIs are the mean ± SEM with n≥6.

(C) MiR-980 inhibition enhances memory acquisition. Three min memory of c155-GAL4>UAS-scrambled and c155-GAL4>UAS-miR-980SP flies was tested after 1, 2, 3, 4, 6 and 12 shock training with a 1min CS+ odor presentation. Shock delivery is schematized below each condition. Statistics: PIs for each shock treatment were tested on different days and were analyzed by two-tailed, two-sample Student’s t-tests for each condition. p<0.05 for 2, 3, 4 and 12 shock treatments. PIs are the mean ± SEM with n=8.

(D) Mir-980 memory retention with a normalized initial PI. Three min PIs of scrambled and miR-980SP flies were normalized with 6 or 4 shocks, respectively during CS+ odor exposure. Memory was tested at 3min, 1, 3, 9 and 24h on different days. Statistics: PIs were compared using two-tailed, two-sample Student’s t-tests for each time point. p<0.05 for 3h memory. PIs are the mean ± SEM with n=8.