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. Author manuscript; available in PMC: 2016 Nov 10.
Published in final edited form as: Cell Rep. 2016 Feb 11;14(7):1698–1709. doi: 10.1016/j.celrep.2016.01.040

Figure 2. Memory enhancement occurs from inhibiting miR-980 in multiple areas of the CNS during adulthood.

Figure 2

(A) Memory enhancement occurs from miR-980 inhibition during adulthood. The c155-GAL4 expression was modulated during development and adulthood using tub-GAL80ts, with temperature shifts schematized below the bar graph. Flies expressing the scrambled control sequence using c155-GAL4; tub-GAL80ts were used as the control for 3h aversive memory. Statistics: PIs were analyzed by two-tailed, two-sample Student’s t-tests for each condition. p<0.01 for flies kept at 18°C during development and 30°C during adulthood and for flies kept at 30°C during both development and adulthood. PIs are the mean ± SEM with n≥10.

(B) MiR-980SP spatial mapping. The UAS-scrambled and UAS-miR-980SP flies were crossed to a battery of GAL4 lines that drive expression in specific populations of neurons. The c155-GAL4 driver was used as the positive control. The GAL4 drivers used and their abbreviated expression domains are shown below the graph. CNS: central nervous system, ORn: olfactory receptor neurons, Pn: projection neurons, APLn: anterior paired lateral neuron, MBn: mushroom body neurons, DPMn: dorsal paired medial neuron, DAn: dopaminergic neurons, CC: central complex, MB-V2n: mushroom body V2 neuron, In: inhibitory neurons, OL: optic lobe. Statistics: PIs were analyzed by two-tailed, two-sample Student’s t-tests for each driver compared to the scrambled crosses. p<0.05 for miR-980SP crossed to GH146-GAL4, c316-GAL4, TH-GAL4, MZ604-GAL44; p<0.01 for c155-GAL4, Or83b-GAL4, NP2492-GAL4; and p<0.0001 for OK107-GAL4. PIs are the mean ± SEM with n≥16.

(C) Mir-980SP expression in the MB using another GAL4 driver, R13F02-GAL4, improved 3h memory consistent with OK107-GAL4 results. Flies were conditioned with 3 shocks during the 1min CS+ odor representation to avoid ceiling scores. Statistics: PIs were analyzed by two-tailed, two-sample Student’s t-test. p<0.05. PIs are the mean ± SEM with n=8.

(D) Overexpression of miR-980 in MB impairs 3h memory. Statistics: Three hour olfactory memory of R13F02-GAL4>UAS-miR-980 flies was compared to GAL4-only and UAS-only controls using one-way ANOVA followed by Bonferroni’s post-hoc tests. PIs are the mean ± SEM with n=12. p<0.01.