Skip to main content
. Author manuscript; available in PMC: 2016 Nov 10.
Published in final edited form as: Cell Rep. 2016 Feb 11;14(7):1698–1709. doi: 10.1016/j.celrep.2016.01.040

Figure 6. Overexpression of A2bp1 potentiates memory; decreasing A2bp1 reverses the memory enhancement due to miR-980SP expression.

Figure 6

(A) Adult-specific overexpression of the A2bp1 RN isoform increases 3h memory. MB specific expression of UAS-A2bp1(RN) was driven by R13F02-GAL4 in the presence of Gal80ts to restrict transgene expression to adulthood. Flies were kept at 18°C throughout development and adulthood (left) or at 18°C during development and switched to 30°C after hatching (right) to induce UAS-A2bp1 overexpression. A2bp1 overexpression in the MB significantly improved memory compared to UAS-only and GAL4-only controls. Statistics: PIs were analyzed using one-way ANOVA followed by Bonferroni’s post-hoc tests. PIs are the mean ± SEM with n=20. p<0.01.

(B) Adult-specific overexpression of the A2bp1-RE isoform increases 3h memory. MB specific expression of a second A2bp1 isoform, UAS-A2bp1(RE), during adulthood also enhanced 3h memory. Statistics: PIs were analyzed using one-way ANOVA followed by Bonferroni’s post-hoc tests. PIs are the mean ± SEM with n=15. p<0.01.

(C) A2bp1 is genetically downstream of miR-980. The UAS-A2bp1 RNAi and UAS-miR-980SP transgenes were combined with a UAS-scrambled transgene in order to obtain flies with the same number of UAS-elements as in the UAS-A2bp1 RNAi; UAS-miR-980SP experimental group. The UAS-scrambled transgenes inserted at the attP40 and attP2 sites were combined together and used as the control. Expression was driven by c155-GAL4. Three hour PIs of UAS-A2bp1 RNAi;UAS-miR-980 SP expressing flies were significantly lower than the scrambled control and miR-980SP-expressing flies, but not significantly different from A2bp1 RNAi-expressing flies. Statistics: Scores were analyzed using one-way ANOVA followed by Bonferroni’s post-hoc tests. PIs are the mean ± SEM with n=36 for the double scrambled control and n=21 for all other groups. p<0.0001.