Figure 2. P-selectin mediates the SLY-induced PNC formation.

(A) S. suis culture supernatant or rSLY protein (1 μg/mL)-induced CD62P release from platelets in human blood was assessed by flow cytometry. Representative histograms of the MFI of CD62P are shown in the left panel. (B) SLY-induced PNC formation in human blood was assessed in the presence of anti-CD62P blocking antibody (10 μg/mL) or an isotype-matched control antibody (10 μg/mL). THB and PBS are the negative controls for culture supernatant and proteins, respectively. Data in (A) right panel and (B) are given as the mean ± SD of three independent experiments from three different blood donors. **P < 0.01; ns, no significance; 05ZYH33, wild type strain; ∆sly, isogenic sly mutants; Sup, supernatant; rSLY, recombinant SLY.