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. 2016 Nov 10;6:36787. doi: 10.1038/srep36787

Figure 6. rPLY or rSLO-induced PNC formation via pore formation-dependent Ca2+ influx.

Figure 6

(A) The cholesterol (100 μg/mL) effect on rPLY (0.8 μg/mL) or rSLO (1.5 μg/mL)-induced PNC formation. (B) The EGTA (3 mM) effect on rPLY (0.8 μg/mL) or rSLO (1.5 μg/mL)-induced PNC formation. PBS is the negative control for recombinant proteins. Cholesterol and EGTA were dissolved in ethanol and H2O, respectively. Data in A and B are given as the mean ± SD of three independent experiments from three different blood donors. *P < 0.05; **P < 0.01; ***P < 0.01; ns, no significance; rSLY, suilysin; Cho, cholesterol; 0.8 + Cho, 0.8 μg/mL of rPLY added to cholesterol; 1.5 + Cho, 1.5 μg/mL of rSLO added to cholesterol.