Figure 2. DSBs repair post-irradiation is facilitated by sleep in female flies.

(a,b) The 4 experimental groups and the amount of sleep before collection in IRR + S and IRR + SD flies. The amount of sleep (min) is shown for consecutive 30 min periods. Yellow and black bars indicate day and night, respectively. (c) Representative images from single layers containing mushroom body cells. Intensity from the H2AV (top), nslGFP (middle) and combined (bottom, nslGFP green, H2AV red) are shown. Scale bar = 1 μm. Bottom row, clear areas are the nuclei, opaque areas are the non nuclear regions, i.e. regions whose background H2AV and nlsGFP signal is below the threshold set for nuclei (see Methods for detail). Outlined with a light blue contour are H2AV foci whose H2AV/background H2AV ratio exceeds threshold, and whose size is >0.2 um³. (d) Percentage of nuclei containing H2AV foci across the fly brain in a non-irradiated control group (C, n = 10), IRR (n = 9), IRR + S (n = 10), and IRR + SD (n = 6) flies. *p < 0.05 with Kruskal-Wallis test followed by Mann-Whitney test. Bars indicate standard error.