Mice were weighed and then subjected to scald injury. Mice were pretreated with 10 mg/kg amitriptyline or saline vehicle. Twenty-four hours after burn injury, (A) weight change, (B) wet spleen mass, and (C) spleen WBC were recorded. Spleen mass was normalized to sham levels and percent of sham level is depicted. The sample size was 16 to 17 per group. Data are expressed as the mean ± SEM. Statistical comparisons were made using Student t test. *, P <0.05 as compared with scald group. Dotted line indicates sham levels. AMIT indicates amitriptyline; WBCs, white blood cells.