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. Author manuscript; available in PMC: 2016 Nov 10.
Published in final edited form as: Shock. 2016 Nov;46(5):541–548. doi: 10.1097/SHK.0000000000000648

Fig. 7. Asm heterozygous mice have blunted autophagy and decreased Bcl-2 expression.

Fig. 7

Bone marrow neutrophils from WT and Asm mice were cultured in complete media for 48 h. Samples underwent lysotracker G (LTG) analysis and (A) percentage LTG expressing neutrophils, and (B) LTG mean fluorescence intensity (MFI) determined. Western Blot was utilized to determine protein concentrations of (C) p62, LC3, and Bcl-2. Quantification of (D) p62 and (E) Bcl-2 of the indicated neutrophils. Sample size was four mice per group and representative of two experiments. Data are expressed as the mean ± SEM. Statistical comparisons were made using Student t test. *, P <0.05 as compared with WT group.