Fig. 2. Antibiotic gavage decreases gut bacterial load and increases colon permeability.
Mice underwent germ reduction by antibiotic gavage as described in the Methods. Isolated cecal stool samples from untreated or antibiotic-treated (ABX) mice were cultured under (A) anaerobic or (B) aerobic conductions and CFU numbers determined. Untreated or ABX mice underwent either (C) FITC dextran gavage to determine total intestinal permeability or (D) intestinal ligation proximal to the cecum and FITC dextran cecal injection to determine colon permeability. Sample size is 8 per group. *P<0.05, compared with saline as determined by Student t test.