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. Author manuscript; available in PMC: 2016 Nov 10.
Published in final edited form as: Curr Top Dev Biol. 2016 Jan 27;117:113–139. doi: 10.1016/bs.ctdb.2015.11.028

Figure 6.

Figure 6

Redundant actions of Gpr124 and Norrin in maintaining BBB integrity. Coronal sections through the cortex and thalamus of postnatal day (P)6 mice with the indicated genotypes. Pdgfb-CreER recombines specifically in ECs. 4-Hydroxytamoxifen (4HT) was delivered by intraperitoneal injection at P3–P4. Intravascular Sulfo-NHS-biotin leaks from the choroid plexus in the lateral ventricles in all brain samples (upper panels), but it leaks extensively into the brain parenchyma only in the sample that is deleted for both Gpr124 and Ndp (upper right panel). Intracerebral capillaries in the WT brain (lower left) and in the brains from mice with either one functional copy of Gpr124 or one functional copy ofNdp (lower middletwo panels) expressclaudin5(a hallmark ofCNS vasculature) and suppress plasmalemma vesicle-associated protein (PLVAP; a hallmark of peripheral vasculature). Deletion of both Gpr124 and Ndp reverses this expression pattern (lower right panel). Scale bar, 200 μm. From Zhou and Nathans (2014).