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. 2016 Aug 19;12(11):2038–2053. doi: 10.1080/15548627.2016.1217371

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© 2016 Sushil Devkota, Hyobin Jeong, Yunmi Kim, Muhammad Ali, Jae-il Roh, Daehee Hwang, and Han-Woong Lee. Published with license by Taylor & Francis.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 4. — EI24 degrades RING domain-containing proteins. (A, D) EI24-mediated degradation of GFP-tagged TRIM family proteins was detected using immunoblotting. MID2, TRIM2, mouse TRIM3, TRIM4, TRIM5, TRIM6, TRIM8, TRIM20, TRIM21, and mouse TRIM26 were tested. (B) EI24 degrades MID2 using autophagy. EI24-mediated degradation of MID2 could be rescued by treatment with BAF (10 nM, 6 h). (C) Protein levels of FBXO7 and STUB1 lacking RING domains were examined for their susceptibility to EI24-mediated degradation. (E) The susceptibility of the RING domain-containing E3 ligases TRAF6, PARK2, XIAP, BIRC2, BIRC3, and MDM2 to EI24-mediated degradation.