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. 2016 Aug 19;12(11):2183–2196. doi: 10.1080/15548627.2016.1219013

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© 2016 Taylor & Francis

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Figure 2. — Cellular localization of GFP-LC3 and PEBP1. HeLa cells were transiently transfected with plasmids expressing GFP-tagged LC3 using Lipofectamine 2000. The cellular localization of GFP-LC3 and PEBP1 in control or starved cells was analyzed under a confocal microscope (Olympus FV1000). Endogenous PEBP1 was detected by immunocytochemistry using anti-PEBP1 and TRITC-conjugated secondary anti-rabbit IgG antibodies. Nuclei were stained with Hoechst 33342. Enlargement is a selected area of the merged image under starvation conditions.