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. 2016 Aug 19;12(11):2145–2166. doi: 10.1080/15548627.2016.1217369

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© 2016 The Author(s). Published with license by Taylor & Francis Group, LLC

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 7. — Etf-1 activates PtdIns3K and colocalizes with ATG5 and LC3. (A) RF/6A cells were transfected with Etf-1-GFP or GFP control plasmids, and the PtdIns3P amount was determined by competitive ELISA at 2 d p.t. Data are presented as the mean ± standard deviation of triplicate assays. *, Significantly different by the Tukey HSD test (P < 0.05). (B and C) Etf-1 colocalizes with ATG5 and LC3. HEK293 cells cotransfected with GFP-ATG5 and Etf-1-DsRed (B) or GFP-LC3 and Etf-1-DsRed (C) were treated at 1 d p.t. with 10 nM BAF for 16 h prior to fixation. Merged/DIC, fluorescence image merged with DIC image. Scale bars: 10 μm. (D) The percentage colocalization of Etf-1 with ATG5 or LC3 was analyzed using Pearson correlation coefficients with ImageJ software. Results were average values of 10 to 20 cells per group ± standard deviation from 3 independent experiments.