Figure 10.

Etf-1 interacts with the RAB5-class III PtdIns3K complex. Co-immunoprecipitation of native Etf-1 with endogenous RAB5, BECN1, and PIK3C3/VPS34. Uninfected (THP) or E. chaffeensis-infected (Ech) THP-1 cells at 2 d p.i. were lysed in modified lysis buffer and immunoprecipitated (IP) with rabbit anti-Etf-1, BECN1, or PIK3C3, or mouse anti-RAB5 IgG cross-linked to protein A/G-magnetic beads for 2 h. Bound proteins were eluted and subjected to western blotting. Arrows indicate the target proteins. *, IgG heavy or light chains. The number under the figure is the density ratio relative to ACT/actin, with uninfected cells set as 1. The absence of a number indicates an infinite ratio (i.e., the protein was absent in the control). Images were representative of 3 experiments with similar results.