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. 2016 Nov 10;13:32. doi: 10.1186/s12014-016-9131-z

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Separation of SP cells from the HO8910 pm cell line and verification of their stemness. a Detection of SP cells from high-grade serous ovarian cancer cells lines. Cells were stained with the dye Hoechst 33342 and analyzed by flow cytometry. The ratio of SP cells was highest in HO8910 pm cells. b The spheres that formed after 21 days of culturing in an ultra-low attachment plate were much larger of SP cells than MP cells. c More spheres formed of SP cells than MP cells. d The mRNA expression of Oct4 and Nanog was up-regulated in SP cells. e SP cells were arrested in G1 stage