Table 2.

Relative intensities of proposed N-glycan structures in SP cells and MP cell

No.	Type	Oberserved m/z (M ± Na)	Proposed structure	SP relative intensitymean (n = 5)	RSD (%)	MP relative intensitymean (n = 5)	RSD (%)	Ratio SP/MP	Change (SP/MP)	Composition
1	High mannose	1257.5		0.1235	7.83	0.1538	7.36	0.80**	Down	H5N2
2		1419.6		0.3051	4.16	0.3054	2.63	1.00	Up	H6N2
3		1581.7		0.1362	4.94	0.1304	5.19	1.04	Up	H7N2
4		1743.8		0.1641	5.43	0.1545	2.61	1.06	Up	H8N2
5		1905.8		0.0382	10.92	0.0319	9.08	1.20*	Up	H9N2
6		2067.9		0.0043	26.10	0.0024	12.27	1.74**	Up	G1H9N2
7		2229.9		0.0007	37.67	0.0005	41.18	1.45	Up	G2H9N2
8	Hybrid	1282.5		0.0372	15.05	0.0350	12.52	1.06	Up	H3N3F1
9		1298.6		0.0308	11.24	0.0330	9.10	0.93	Down	H4N3
10		1444.6		0.0108	26.90	0.0127	8.19	0.85	Down	H4N3F1
11		1460.8		0.0165	32.28	0.0316	12.45	0.52**	Down	H5N3
12		1622.7		0.0092	26.98	0.0107	22.17	0.86	Down	H6N3
13	Complex	1339.6		0.0339	26.80	0.0366	37.17	0.93	Down	H3N4
14		1485.6		0.0219	24.14	0.0136	4.33	1.61**	Up	H3N4F1
15		1501.6		0.0110	14.54	0.0082	11.88	1.35*	Up	H4N4
16		1542.6	Or	0.0219	3.77	0.0136	10.59	1.61***	Up	H3N5
17		1647.7		0.0058	21.47	0.0047	20.87	1.22	Up	H4N4F1
18		1688.7	Or	0.0043	21.63	0.0021	39.72	2.04**	Up	H3N5F1
19		1704.7	Or	0.0079	16.73	0.0041	7.03	1.90**	Up	H4N5
20		1809.8		0.0110	14.70	0.0110	20.01	1.01	Up	H5N4F1
21		1850.8	Or	0.0011	56.44	0.0007	27.40	1.49	Up	H4N5F1
22		1866.8		0.0024	20.34	0.0013	47.41	1.91*	Up	H5N5
23		2012.9		0.0010	46.15	0.0008	51.52	1.38	Up	H5N5F1
24		2175.0		0.0008	26.05	0.0008	33.00	1.02	Up	H6N5F1
25		2377.1		0.0005	52.38	0.0005	34.80	0.90	Down	H6N6F1

Values of relative intensity mean represent mean of 5 replicates; symbols for proposed structures: GlcNAc, , Man, , Glc, , Fuc, , Gal,

H hexose, N N-acetyl hexosamine, F fucose, G glucose