Fig. 2.

Impact of drought on stress markers and reactive oxygen species formation in leaves and roots of maize. a-b Proline steady state levels in leaves (a) and roots (b) in control conditions (black) and after restriction of water supply (white) for indicated time points (n = 5). c In situ staining of hydrogen peroxide formation in leaves of drought-stressed maize (n = 3). d-e Oxidation of the glutathione pool (GSSG/GSH ratio) in leaves and roots of drought-stressed maize (n = 5). f-i Impact of drought stress on transcription (f, g, n = 3) and enzymatic activity (h, i, n = 4) of glutathione reductase (GR) in leaves (f, h) and roots (g, i) of maize. Data are means ± SD of three to five individual replicates. Asterisks indicates statistical differences as determined by the unpaired t-test (*, 0.05 ≥ p > 0.01; **, 0.01 ≥ p > 0.001; ***, p ≤ 0.001)