Table 4.
Identification of polyphenolic compounds in A. microcarpus flowers ethanolic extract by HPLC-DAD-ESI/MS analysis
Peak | Rt (min) | λmax (nm) | Pseudomolecular ion [M-H]− (m/z) | MS2 (m/z), (%) | Tentative identification |
---|---|---|---|---|---|
1 | 5.9 | 326 | 353 | 191 (100) | 3-O-caffeoylquinic acid |
353 (94) | |||||
179 (71) | |||||
135 (48) | |||||
173 (9) | |||||
2 | 8.3 | 326 | 353 | 191 (100) | 5-O-caffeoylquinic acid |
3 | 15.4 | 346 | 447 | 447 (100) | Luteolin-6-C-glucoside |
357 (98) | |||||
327 (97) | |||||
285 (25) | |||||
429 (20) | |||||
4 | 19 | 340 | 447 | 447 (100) | Luteolin-O-hexoside |
285 (40) | |||||
5 | 21.5 | 338 | 447 | 285 (100) | Luteolin-7-O-glucoside |
6 | 23.9 | 338 | 489 | 285 (100) | Luteolin O-acetylglucoside |
7 | 26.8 | 345 | 285 | 285 (100) | Luteolin |
8 | 27.9 | 324 | 593 | 285 (100) | Luteolin O-deoxyhexosylhexoside |
9 | 29.9 | 288 | 271 | 151 (100) | Naringenin |
119 (56) | |||||
10 | 30.2 | 338 | 269 | 269 (100) | Apigenin |
151 (36) | |||||
11 | 30.8 | 348 | 299 | 300 (100) | Methyl-luteolin |
285 (88) |