Fig. 5.

Gene content and organization around the putative mating type genes of P. rhodozyma. a Genomic organization of the DNA regions with putative mating pheromone receptors (Ste3-1 and Ste3-2), pheromone precursors (Mfa1 and Mfa2) and b homeodomain transcription factors (HD1 and HD2). Genes are indicated by arrows showing the direction of transcription. Arrows in grey indicate putative mating related genes while those in white depict additional genes apparently unrelated to mating. Hyp1-2 are hypothetical predicted proteins of uncertain function. The end of a scaffold is indicated by a solid vertical bar. The contiguity of the scaffolds harboring the two sets of pheromone and receptor genes was confirmed by PCR with primers MP100 and MP103. c Sequence alignment of the putative pheromone precursors from P. rhodozyma (CBS 7918^T) and C. neoformans var. neoformans (Cnn Mfα1 - XP_570122.1; Cnn Mfa1- AAG42766.1). The predicted mature pheromones are shaded in grey and the CAAX-motifs for C-terminal processing are underlined