Table 2.

Mapping of essential MIAPPE attributes to ISA-Tab structures in Phenotyping Configuration: Basic, Field and Greenhouse

Checklist section	ISA-Tab level	Checklist attribute	ISA-Tab structure	Basic	Field	Greenhouse
General metadata	Investigation	Unique identifier		●	●	●
		Title		●	●	●
		Description		●	●	●
Timing and location	Study	Timing  Start of experiment (date)  Duration (days/months/year)	Characteristics	●	●	●
		Experiment location  Geographic location		●	●	●
Biosource	Study	Organism (taxon)	Characteristics	●	●	●
		Infraspecific name		●	●	●
		Seed origin		●	●	●
Environment	Study	Growth facility (growth chamber, GC/greenhouse, GH/open top chamber, OTC/experimental garden/field)	Characteristics	●	●	●
		Aerial conditions  Air humidity (moisture)  Daily photon flux (light intensity)  Temperature (°C):   Average day temperature    Average night temperature	Protocol “Aerial conditions” with parameters		●	●
		Rooting conditions  Rooting medium: aeroponics/hydroponics (water-based, solid-media based)/soil type (sand, peat, clay, mixed, etc.)  pH  For field:   Plot size   Sowing density  For greenhouse:   Container type   Container volume   Container dimensions   Number of plants per container	Protocol “Rooting” with parameters		●	●
		Nutrients  For soil:   Extractable N content per unit ground area before fertiliser added   Type and amount of fertiliser added,	Protocol “Nutrition” with parameters		●	●
		Watering  For soil:   Range in water potential (MPa)   Irrigation from top/bottom/drip irrigation	Protocol “Watering” with parameters		●	●
Treatments	Study or Assay	All interventions being part of the experiment	Factor or Protocol with parameters		□	□
Experimental design	Study	Experimental units and their grouping (into blocks, superblocks etc.)	Characteristics, Factor, Protocol “Sampling” with parameters		●	●
Sample collection, processing, management	Assay	Plant body of interest (organ)	Characteristics	●	●	●
Observational variables	Assay	Phenotypic variables  Trait  Method  Scale		●	●	●
		Environmental variables  Trait  Method  Scale		□	□	□
Observations	Assay	Raw data	Raw data file	□	□	□
		Derived data	Derived data file	●	●	●

●—included in the ISA-Tab configuration; □—not included in the configuration, specific per experiment