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Primer sequences of sepecific genes
| Primer name | Sequence(5' to 3') | |
|---|---|---|
| Sox 9 | F | GGAGATGAAATCTGTTCTGGGAATG |
| R | TGAAGGTTATCTGCTGGTGTTCTGA | |
| ACAN | F | TCTACCGCTGCGAGGTGA |
| R | TGTAATGGAACACGATGCCTTT | |
| Col2a1 | F | GGAAGAGTGGAGACTACTGGATTGAC |
| R | TCCATGTTGCAGAAAACCTTCA | |
| Nanog | F | ACAACTGGCCGAAGAATAGCA |
| R | GGTTCCCAGTCGGGTTCAC | |
| Oct 4 | F | GGAGGAAGCTGACAACAATGAAA |
| R | GGCCTGCACGAGGGTTT | |
| β-actin | F | CGAGAAGATGACCCAGATCATG |
| R | ACAGCCTGGATAGCAACGTACA | |
