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A portion of the FGF23 gene was amplified from genomic DNA obtained from the proband and available family members as described in Material and methods section, using the forward primer 5′-CTCAACGCCCTAAGAACTG-3′ and the reverse primer 5′-CCTCATTTCAGCAAGCATCA-3′. The G-to-A nucleotide change, which causes the R176Q mutation, introduces a site for the restriction endonuclease TseI (*), leading to the generation of two additional fragments that are 334 bp and 203 bp in length.
