Fig. 3.

Modeling a 3.5 Å cryoEM map of VipA/B (Kudryashev et al., 2015). (A) The 3.5 Å reconstruction (EMDB2699) of VipA/B, the contractile sheath of the type VI secretion system. (B) A model of the two protein components, built using Rosetta de novo building followed by optimization with RosettaCM (PDB 3J9G). (C) A close-up view of the asymmetric unit model, shown in density. The two panels on the right show regions of relatively low local resolution; Rosetta de novo allowed placement of the models in these regions.