Figure 3.

Effects of autophagy inhibitors on the cell viability of MPP+-treated astrocytes. (A) The effects of 3-MA on the viability of MPP+-treated cells. Astrocytes were pretreated with 3-MA (10 mM) for 1 h, after which they were treated with MPP+ (400 or 800 µM) for 24 h. (B) The effects of various doses of 3-MA on the viability of MPP+-treated cells. Astrocytes were pretreated with 3-MA (2, 5 or 10 mM) for 1 h, after which they were treated with MPP+ (800 µM) for 24 h. (C) The effects of CQ on the viability of MPP+-treated cells. Astrocytes were pretreated with CQ (0.01 mM) for 1 h, after which they were treated with MPP+ (400 or 800 mM) for 24 h. The cell viability was determined using MTT assays, and absorbance was measured using a microplate reader. Data are presented as the mean ± standard error of the mean of triplicate experiments. *P<0.05 vs. the Con and MPP+-treated astrocytes. MPP+, 1-methyl-4-phenylpyridinium; 3-MA, 3-methyladenine; CQ, chloroquine; Con, control group.