Figure 4.

Effects of 3-MA pretreatment on LC3 II and pAkt protein expression levels in astrocytes treated with MPP+. Astrocytes were pretreated with 3-MA (10 mM) for 1 h, after which they were treated with MPP+ (400 or 800 µM) for 24 h. Protein expression levels of (A) LC3 II and (B) pAkt were detected using western blotting. The relative protein expression levels of (C) LC3 II and (D) pAkt were quantified by calculating OD values. Data are presented as the mean ± standard error of the mean of triplicate experiments. *P<0.05 vs. the control group; ^#P<0.05 vs. the MPP+-treated astrocytes. 3-MA, 3-methyladenine; LC3 II, microtubule-associated protein light chain-3; MPP+, 1-methyl-4-phenylpyridinium; OD, optical density; Con, control.