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. Author manuscript; available in PMC: 2016 Nov 10.
Published in final edited form as: J Urol. 2015 Apr 23;194(3):790–798. doi: 10.1016/j.juro.2015.02.2951

Figure 5.

Figure 5

Analytical validation of NGS assay showing correlation between allelic frequencies from runs at 2 sites. MDA-MB-231 model tumor cell line was spiked into healthy donor blood, resulting in final CTC concentrations of 0 to 8 CTCs/ml blood. Cell line has 3 known somatic variants in BRAF, KRAS and TP53 genes. NGS workflow detected each variant for 17 of 18 positive calls with only 1 false-positive call in test group.