Skip to main content
. Author manuscript; available in PMC: 2017 Sep 8.
Published in final edited form as: Cell. 2016 Sep 8;166(6):1471–1484.e18. doi: 10.1016/j.cell.2016.07.029

Figure 3. Generation and characterization of VH1-2/LC model.

Figure 3

(A) Illustration of genetic modifications involved in the VH1-2/LC model. See text, Figure S3A and S3B for details. (B) HTGTS-rep-seq analysis of VH usage in splenic B cells from VH1-2/LC mice performed as in Fig. 1C. (C) D segment usage in productive IGHV1-2*02 rearrangements in VH1-2/LC mouse model. (D) Length distribution of IGHV1-2*02-associated CDR H3s in VH1-2/LC mouse model. (E) Single cell analysis of VRC01 IgL chain expression in splenic B cells of VH1-2/LC mouse model. Primers for VRC01LC cDNA in the leader exon (L) of IGKV3-20*01 and the Cκ exon are represented by arrows. Gel images are representative of results from two different VH1-2/LC mice. Numbers of VRC01LC+ and VRC01LC− cells are indicated on the pie chart. Other details are in Experimental Methods.