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. Author manuscript; available in PMC: 2017 Sep 8.
Published in final edited form as: Cell. 2016 Sep 8;166(6):1471–1484.e18. doi: 10.1016/j.cell.2016.07.029

Figure 4. Stepwise immunization of the VH1-2/LC mice elicited VRC01-class CD4bs-specific antibodies with neutralizing activity.

Figure 4

(A) Immunization Schema. (B) Characterization of immunogens (varied symbols) for ELISA binding to VRC01 and its germline reverted variant (VRC01gl). (C) ELISA endpoint titers of post-immune sera to three germline-binding VRC01-class probes and their respective CD4bs-knock out (Δ) mutants. Error bars indicate standard deviations. (D) Splenocytes were sorted with eOD-GT6 and ΔeOD-GT6 probes to isolate CD4bs-specific IgG+ B cells. (E) Neutralizing activity of post-immune sera to various HIV-1 Env-pseudoviruses (ID50 titers). n.d., not determined.