Table I.
p38 MAPK and CBP are both miR-22 predicted targets using the TargetScan software.
| Position | Predicted consequential pairing of target region (top) and miRNA (bottom) | Seed match | Sitetype contribution | 3′ pairing contribution | Local AU contribution | Position contribution | Context score | Context score percentile | Conserved branch length | PCT |
|---|---|---|---|---|---|---|---|---|---|---|
| 1211–1217 of CREBBP | 5′ AUUGCAGUGGGUAUUGGCAGCUG | 7merm-8 | −0.161 | 0.005 | −0.019 | 0.073 | −0.10 | 41 | 0.515 | <0.1 |
| 3′ UTR mo-miR-22 | 3′ UGUCAAGAAGUUGACCGUCGAA | |||||||||
| 1283–1289 of CREBBP | 5′ CACAGAGAGUGAGGGGGCAGCUC | 7merm-8 | −0.161 | 0.005 | 0.092 | 0.077 | 0.01 | 3 | 0.086 | <0.1 |
| 3′ UTR mo-miR-22 | 3′ UGUCAAGAAGUUGACCGUCGAA | |||||||||
| 1285–1291 of MAPK14 | 5′ GGCCCCCCCGCCCCCGGCAGCUU | 7merm-8 | −0.161 | 0.067 | 0.114 | 0.030 | 0.05 | 0 | 1.796 | 0.59 |
| 3′ UTR mo-miR-22 | 3′ UGUCAAGAAGUUGACCGUCGAA |
The predicted consequential pairing of target regions are indicated in bold. MAPK, mitogen-activated protein kinase; CREB, cAMP response element binding; CBP, CREB binding protein; AU, adenine purine.