Table 3. PCR assays.

Gene	Full gene name	Accession no.	Forward primer	Reverse primer	Amplicon size (bp)	PCR efficiencya/b*
cuznsod	CuZn superoxide dismutase	BG936553	CCACGTCCATGCCTTTGG	TCAGCTGCTGCAGTCACGTT	141	1.94/1.95
mnsod	Mn superoxide dismutase	DY718412	GTTTCTCTCCAGCCTGCTCTAAG	CCGCTCTCCTTGTCGAAGC	227	1.96/2.06
cat	Catalase	BG935638	CCCAAGTCTTCATCCAGAAACG	CGTGGGCTCAGTGTTGTTGA	101	1.90/1.97
gpx1	Glutathione peroxidase 1	DW566563	GCCCACCCCTTGTTTGTGTA	AGACAGGGCTCCACATGATGA	103	1.85/2.05
gpx3	Glutathione peroxidase 3	DW561212	TTCCCCTCCAATCAGTTTGG	ATCCCCCCTCTGGAATAGCA	123	1.97/2.05
gr	Glutathione reductase	BG934480	CCAGTGATGGCTTTTTTGAACTT	CCGGCCCCCACTATGAC	61	2.03/1.97
g6pd	Glucose-6-Phosphate dehydrogenase	> Contig7869_Atlantic_salmon	CTTTGGGCCAATCTGGAACA	TCCCGGATGATTCCAAAGTC	114	2.08/2.00
nfe2l2	Nuclear factor (erythroid-derived 2)-like 2	BT044699	TCGCTGAAGGAGGAGAAGGA	GTCCCTCAGCAGACGGAAAA	120	1.83/2.05
gclc	Glutamate-Cysteine ligase, catalytic subunit	> Contig16361_Atlantic_salmon	CGTCCTGCTCACCAGGGTTA	GCCCTCTTGGACTGCATTTC	112	1.93/2.00
actb	Beta-actin	BG933897	CCAAAGCCAACAGGGAGAA	AGGGACAACACTGCCTGGAT	92	1.93/20.00
ee1ab	Eukaryotic translation elongation factor 1 alpha B	BG933853	TGCCCCTCCAGGATGTCTAC	CACGGCCCACAGGTACTG	57	2.04/2.03
uba52	Ubiquitin A-52 Residue ribosomal protein fusion product 1	NM_001141291	CCAATGTACAGCGCCTGAAA	CGTGGCCATCTTGAGTTCCT	110	2.04/-

Note:

^*a = Trial 1, b = Trial 2.