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. Author manuscript; available in PMC: 2016 Nov 10.
Published in final edited form as: Methods Enzymol. 2014 Dec 5;550:23–39. doi: 10.1016/bs.mie.2014.10.030

Figure 2.

Figure 2

Self-replicating aptazymes. (a) An aptamer domain (dashed box) is linked to the central stem-loop of the self-replicating RNA enzyme via a connecting stem (open lines). (b) Modification of the central stem-loop to include an aptamer that binds theophylline (theo). The connecting stem has been optimized to maximize activity in the presence compared to the absence of theophylline. (c) The two substrate-recognition domains of the enzyme (SR1 and SR2) can be altered in sequence, so long as complementarity is maintained.