Figure 3. Inhibitors and activators of DMT1-mediated iron uptake.

Results of transport assays performed as described in Figure 1 are shown for indicated agents compared with vehicle control (0.5% DMSO, v/v, bottom panel) or water alone (top panel). Results are from single independent experiments' means ± SEM determined for biological triplicates with similar results obtained on at least two separate occasions. For pertussis toxin (PTX) experiments, cells were treated with 100 ng/ml toxin added to culture medium for at least 24 h prior to assays (*P < 0.01; **P < 0.05).