Table II.

Primers used in amplification refractory mutation system-quantitative polymerase chain reaction.

Primer	Sequence, 5′-3′
Forward (nucleotide position 10373–10398)
Wild-type sequence	GT GACTACAAAAAGGATTAGACT g a A
Wild-type 10398A primer	GT GACTACAAAAAGGATTAGACT c t A
Mutated 10398G primer	GACTACAAAAAGGATTAGACT c t G
Reverse (nucleotide position 10753–10777)
Wild-type sequence	TACTCCAATGCTAAAACTAATCGT C
Antisense strand sequence	G ACGATTAGTTTTAGCATTGGAGTA
Wild-type 10398A primer	G ACGATTAGTTTTAGCATTGGAGTA
Mutated 10398G primer	ACGATTAGTTTTAGCATTGGAGTA

Underlined lowercase bases indicate the mismatches.