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. 2016 Sep 8;12(5):3153–3160. doi: 10.3892/ol.2016.5106

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Copyright: © Tian et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — PCR cloning of LP-15 RCC1 functional domain cDNA. (A) 1% (w/v) agarose gel electrophoresis of LP-15 RCC1 functional domain cDNA. M, DNA Marker DL2000; Lane 1, PCR products of LP-15-RCC1 cDNA. (B) Plasmid pET-32a(+)-LP-15-RCC1 carrying RCC1 functional domain cDNA. M, DNA Marker DL8000; Lane 1, plasmid pET-32a(+)-LP-15-RCC1 extracted from JM109 cells; Lane 2, double restriction enzyme digestion of plasmid pET-32a(+)-LP-15-RCC1 extracted from JM109 cells. RCC1, regulator of chromosome condensation 1; LP-15, Latcripin-15; PCR, polymerase chain reaction.