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. 2016 Sep 2;12(5):3241–3249. doi: 10.3892/ol.2016.5081

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Cancer cell apoptotic in the tumor co-administered with cetuximab and iRGD. (A) Apoptotic cells in the tumor tissues were detected by a TUNEL assay. TUNEL-positive nuclei are stained brown. TUNEL-negative nuclei are stained blue. The figures shown here are representative of the six tumors in each group. Arrows indicate the apoptotic bodies. Magnification, ×400; Scale bars=50 µm. (B) Quantitative analysis of the apoptosis index in each group. The percentage of TUNEL-positive cells counted from 100 randomly selected tumor cells per section. Five sections were counted per tumor. n=6; Error bars, mean ± standard deviation; ***P<0.001. PBS, phosphate-buffered saline; iRGD, internalizing RGD peptide; TUNEL, TdT-mediated dUTP nick end labeling kit.