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Analysis of Notch4 expression in human PC cell lines. The Notch4 expression levels in cellular total protein of PC cell lines and in HPDE6c7 cells were determined by western blotting. Whole-cell extracts of HPDE6c7, MiaPaCa-2, BxPC3, PANC-1 and Capan-1 cells were used for western blot analysis. The level of GAPDH was used as an internal control. The data were obtained from three independent experiments. PC, pancreatic cancer; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.
