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Knockdown of Notch4 in pancreatic cancer cells. Western blot analysis revealed that the expression of Notch4 was highly reduced in Notch4 siRNA-transfected MiaPaCa-2 and PANC-1 cells. GAPDH was used as an internal control to demonstrate equal protein loading. Lane 1, blank control; lane 2, control siRNA; lane 3, Notch4 siRNA. The data were obtained from three independent experiments. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; siRNA, small interfering RNA.
