Figure 5.

Dual inhibition of DDR1 and SFK decreased the tumorigenecity of NPC cells in vitro. (A) Effect of combined 7RH and dasatinib treatment on the survival, progression and apoptosis of CNE2 cells. (A) CNE2 cells were treated with 4 µM 7RH alone, 5 µM dasatinib alone or 7RH ± dasatinib and the expression levels of cell cycle-associated proteins were analyzed by western blotting. (B) The apoptosis of NPC cells treated with 4 µM 7RH and/or 5 µM dasatinib for 48 h and stained with Annexin V and propidium iodide was analyzed using flow cytometry. The apoptosis rates of the cells treated with 7RH ± dasatinib were increased when compared with cells treated with either agent alone. (C) The cell adhesion profile was analyzed by adhesion assays and western blot analysis of p-Pyk2 and p-FAK. Decreased cell adhesion ratios were observed for cells treated with 7RH and 7RH + dasatinib, as quantified using ImageJ software. Data are presented as the mean ± standard deviation of triplicate experiments *P<0.05 vs. control experiments within the same group. NPC, nasopharyngeal carcinoma; DDR1, discoidin domain receptor 1; SFK, SRC family kinase; p-, phosphorylated; ERK, extracellular signal-regulated kinase; 4EBP1, eukaryotic translation initiation factor 4E binding protein 1; MCL-1, myeloid cell leukemia-1; BCL-2, B-cell lymphoma-2; PARP, poly(ADP-ribose) polymerase 1; FAK, focal adhesion kinase; DMSO, dimethyl sulfoxide; FITC, fluorescein isothiocyanate.