Figure 4.

hTERT are direct targets of miR-138. (A) Predicted binding sites for miR-138 in the 3′UTR of hTERT and the mutations in the binding sites are shown. (B) Luciferase assay was performed to confirm the direct regulation of miR-138 on hTERT 3′UTR. **P<0.01 vs. miR-NC. (C) hTERT protein expression in HeLa cells transfected with miR-138 mimic were detected by western blot analysis. β-actin was used as an internal control. hTERT, human telomerase reverse transcriptase; miR-138, microRNA-138; 3′UTR, 3′ untranslated region; wt, wild type; mut, mutant type.