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Primer sequence and annealing temperature of the XIAP and reference genes.
| Gene | Primer (5′→3′) | PCR amplification products size (bp) | Annealing temperature (°C) | |
|---|---|---|---|---|
| XIAP | Sense: | ATGGGATTTGATTTCAAGGA | 256 | 55 |
| Antisense: | GTCCACAAGGAATAAAAACA | |||
| GAPDH | Sense: | ACCACAGTCCATGCCATCAC | 452 | 55 |
| Antisense: | TCCACCACCCTGTTGCTGTA |
XIAP, X-linked inhibitor of apoptosis protein.
