Table I.
Oligonucleotide sequences of the primers used in the present study.
| Primer | Sequence, 5′-3′ | Annealing temperature, °C | Product length, bp |
|---|---|---|---|
| EXT1 | |||
| Forward | 5′-CTTCGTTCCTTGGGATCAAT-3′ | 55.42 | 95 |
| Reverse | 5′-TGCCTTTGTAGATGCTGGAG-3′ | 57.59 | |
| WISP1 | |||
| Forward | 5′-CAAGGCTGGATAACAGCTCA-3′ | 57.59 | 87 |
| Reverse | 5′-TTCCCAAATTGAGATGCAAA-3′ | 53.62 | |
| ATAD2 | |||
| Forward | 5′-CCAGACAGCAGGCTGATAAA-3′ | 57.59 | 137 |
| Reverse | 5′-ACGCACTTCAACATCACCAT-3′ | 58.10 | |
| TSPYL5 | |||
| Forward | 5′-TGCACAAGTCTCCCTGCTAC-3′ | 59.68 | 87 |
| Reverse | 5′-CAGAGGCCAACATGAAGAGA-3′ | 57.22 | |
| MTDH | |||
| Forward | 5′-TGCCGCCAATACTACAAGAG-3′ | 57.69 | 105 |
| Reverse | 5′-GTTTGGGAGATTCCCAGCTA-3′ | 56.90 | |
| CCNE2 | |||
| Forward | 5′-CGGCCTATATATTGGGTTGG-3′ | 55.44 | 106 |
| Reverse | 5′-ACGGCTACTTCGTCTTGACA-3′ | 59.04 | |
| GAPDH | |||
| Forward | 5′-ATGGAGAAGGCTGGGGCT-3′ | 60.29 | 125 |
| Reverse | 5′-ATCTTGAGGCTGTTGTCATACTTCTC-3′ | 60.85 |
EXT1, exostosin glycosyltransferase 1; WISP1, WNT1 inducible signaling pathway protein 1; TSPYL5, TSP-like 5; MTDH, metadherin; ATAD2, ATPase family, AAA domain containing 2; CCNE2, cyclin E2; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.