Figure 4.
NAD+ deficiency disrupts retinal energy homeostasis and can be rescued with exogenous NMN. (A–C) Wild-type mice (129S1/SvImJ) receiving intraperitoneal injections of 300 mg/kg NMN were more resilient against light exposure (n=8/group; 2-way mixed ANOVA with Bonferroni post-hoc test) compared to vehicle-treated mice. (D) Representative electron microscopy images of retinas from 4-week old Nampt−rod/−rod mice revealed mitochondria that are rounded, disorganized, and with loss of cristae compared to NamptF/F mice. (E) Nampt−rod/−rod retinas also exhibited significant disruption of numerous metabolic pathways on metabolite set enrichment analysis. Graphs depict mean ± S.E.M. (A–C) (* p < .05).